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Category:Software stubsBrief communication: positional information encoded in the distal-tip cell domains in posterior mesoderm of the chick embryo.
In the chick, an anterior-posterior positional information is decoded by Hensen’s node (HN) mesoderm and secondary mesoderm in posterior mesoderm. HN and secondary mesoderm are separated in the anterior half of chick tailbud from rhombomere 3-8, but they overlap in the posterior half. To analyze this positional information, the spatial patterns of HN and secondary mesoderm were compared by transplanting rhombomere 4 into ectoderm in isolated chick tails. The results showed that in posteriorly-transplanted tailbuds, the polarities of HN and secondary mesoderm were perfectly correlated; that is, the posterior-lateral edge of HN mesoderm was parallel to the posterior-lateral edge of secondary mesoderm in the tailbud. This is consistent with the previous findings that HN and secondary mesoderm are generated by the same activity, and secondary mesoderm extends through HN mesoderm. Therefore, we conclude that the secondary mesoderm in posteriorly-transplanted tailbuds represents the posterior-lateral portion of HN mesoderm. We also found that all the HN precursor cells (HPCs) in anterior-transplanted tails had different distal-tip cell markers, suggesting the HN precursors are generated from distinct domains of distal-tip cell. In addition, we found that the HPCs (rhombomere 4) and the secondary mesoderm (rhombomere 5-8) were separately generated from their respective domains, in agreement with the previous finding that the HN is composed of two domains, A and B. These results suggest that different domains of the secondary mesoderm in posterior-transplanted tailbuds represent its anterior-lateral and posterior-lateral domains in the chick embryo, respectively. It will be of interest to investigate the molecular basis of this positional information.The impotence sufferers are in a sorry situation. You must seek advice from your doctor about the extent of your damage. Most men who are affected by this condition feel http://sketcheny.com/?p=4148
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